| 1. | Virul isolation had been done for the 23 positive samples by using cam or cef . we only got 11 strains of ibdv
在此基础上设计的nested - pcr则大大提高了阳性检出率(提高52 . 1 ) 。 |
| 2. | " in the past four weeks , about 87 per cent of positive samples were influenza a mostly h3n2 while the remaining were influenza b virus , " he said
于过去四周大约百分之八十七流感病毒属甲型流感大多属h3n2 ，馀下的则属乙型流感。 |
| 3. | In this paper , the svm is trained on 4097 positive samples made up of white blood cell nuclei pixels and 2910 negative samples ( some red blood cells and background pixels )
本文将分割问题转化为分类问题，利用支持向量机( svm )实现血液细胞核彩色图像分割。 |
| 4. | After an agonizing year of negative results , we found the first influenza - positive sample in 1996 , a lung specimen from a soldier who died in september 1918 at fort jackson , s . c
经过一整年负面结果的痛苦折磨之后，我们在1996年找到了第一个带有流感病毒的样本，那是来自1918年9月死于南卡罗来纳州杰克逊堡的一名士兵的肺组织。 |
| 5. | Using a set of primers shared by endogenous and exogenous alvs ( a > b > c , d , e ) , gp85 gene was amplified by polymerase chain reaction ( pcr ) from dna extracted from common cef and exogenous alv positive samples
根据已经发表在ncbi的alv - a 、 b 、 e亚群的序列情况，分析多态性酶切位点，运用不同的限制酶bgl ， bamh ， ssp进行分型。 |
| 6. | The ratio of right predictive number of positive sample to wrong predictive number of positive sample is used as the fitness of the improved genetic algorithm , because the positive sample is dramatically small with high unbalanced distribution in practical binary classification
针对现实的二值分类中正向样本数极少且分布不平衡的特点,提出利用正确正向分类结果与错误正向分类结果的比值作为遗传算法的适应度函数。 |
| 7. | Blood samples from 12 , 000 hong kong citizens were collected and screened for the presence of the sars - cov antibody using enzyme - linked immunosorbent assay ( elisa ) . positive samples underwent immunoflourescence assay ( ifa ) and western blot for confirmation
经初步酵素连结免疫吸附分析法( elisa )测试呈阳性的样本更会透过免疫萤光法分析法( immunofluorescenceassay ) ，及西方点墨法( westernblot )化验以作最终确定抗体的存在。 |
| 8. | All the samples with typical potato spindle tuber viroid ( pstvd ) symptoms collected from the fields in the northeast region of china were detected by the r - page method . the positive samples were verified . then , two - dimensional page and rt - pcr techniques were adapted in deleting pstvd
对采自中国东北佳木斯、大庆等地区具有典型马铃薯纺锤块茎类病毒( pstvd )症状的薯块样品进行往返电泳( r - page ) ，确定含马铃薯纺锤块茎类病毒的阳性样品。 |
| 9. | Two plasmids , which contain hbv specific dna fragment and hsv1 dna fragment , were amplified by solid phase two loci pcr and detected by enzymatic indicator system on a gene chip that was constructed by primer immobilization and modified with thiol group on chip surface . for building detection technology by dna chip in clinical , the virus genomes were extracted from the clinical positive samples by one - step nucleic acid extraction
采用已建立的芯片制备方法，在该六种质粒中选择含有hbv与hsv1两种病毒dna序列的质粒为模板，进行同相两重pcr ，采用芯片酶学枪测对固相两重pcr的扩增结果进行检测，得到良好的芯片酶学检测结果。 |
